Original Research Paper

A Model Of Type Ii Diabetes In Rat Using Low Fructose Diet And Alloxan

Abstract :

This study aimed at developing a type II diabetic animal model with low mortality, suitable for chronic studies using low fructose diet and a single injection of alloxan.Thirty male Wister rats weighing between 150g-200g were equally divided into five groups. Group 1 (control) drank distilled water while groups 2 to 5 rats drank 20%w/v solution of fructose freely for 7days. Thereafter, groups 2, 3 and 4 rats received 20mg/kg-i.v, 40mg/kg-i.v and 150mg/kg-i.p alloxan respectively. Fasting Blood Glucose (FBG) was measured after fructose administration then 24hours, 6 weeks and 12 weeks after alloxan injection. Groups 2, 3 and 4 showed significant increase in FBG (124mg/dl, P<0.05; 286mg/dl, p<0.01; 419mg/dl, p<0.001) compared with control (60mg/dl). These findings showed that low fructose diet with a single injection of alloxan induces a diabetic condition of less severity, depending on the dose and/or route of administration of alloxan.

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